To clearly outline this issue, Vancomycin-resistant enterococci organisms were analyzed and comparisons evaluated using two Chromogenic (CHM-Gn) media. The outcome of the experiment was impressive where the two media, CHM-Gn â€œAâ€ and CHM-G â€œBâ€ produced exemplary results after they were enriched overnight and incubated for 48-hours. Both media had 98% (53/54) sensitivity and an average of 95.5% (196/205) specificity. Biochemical testing was used to further identify species whose color was difficult to pick especially on CHM-Gn â€œAâ€. A light microscope was also used to observe the easily unidentifiable species to the naked eye.
Media â€œAâ€ and â€œBâ€ produced exemplary results after they were enriched overnight and incubated for 48-hours. Both media had a 98% (53/54) sensitivity, 95.5% (196/205) specificity. Both species Enterococcus solitarius and Enterococcus hirae were unable to grow on both media. Enterococcus gallinarum was able to grow as tiny bluish colonies on CHR Upon dilution and 48-hours incubation. Both media revealed a typical colony appearance as described by the manufacturers (Table A). The presence of foul bacteria on the plate resulted in low sensitivity.
After 24 hours of incubation unexpectedly, with seven color shades at colony edges for VREfaecium and six for VREfaecalis. Two similar colors were observed in the center of VREfaecium and VREfaecalis colonies, thereby complicating definite VRE species identification. Generally, after 24hrs cultivation, VREfaecalis presented a stable green color while VREfaecium had a different mixture of colors. VREfaecium colonies were seen as red most of the time. Colonies on â€œAâ€ expressed superior growth in comparison to â€œBâ€ colonies leading to untimely uncovering and identification of VREfaecium and VREfaecalis in 24hrs. Xylose fermentation test confirmedÂ E. gallinarumÂ species growth on media â€œAâ€ and â€œBâ€. Yeast also cultivated well as white (on â€œBâ€) and pale green (on â€œAâ€) media as that had led to misleading colony colors.
Comparative analysis revealed unacceptable results after foul bacteria were cultured, hence one of the VREÂ sppÂ undetected from the two media. This was as a result of plating saline diluted stool samples on media â€œAâ€ and â€œBâ€ Comparable screening of over 200 stool specimens on media â€œAâ€ and â€œBâ€ was analyzed, resulting in a colonization index of 0.21(21%) for both media (Table A). The MLVA (Multiple Locus Variable Analysis) was used to determine VREfaeciumâ€™s genetic relations among its isolates(Over 40 isolates), where a summation of six unlike MLVA variety was arrived at (5). These varieties were expressed in both media.
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