The Mussels were sampled in three bays namely: Guanabara Bay, Sepetiba Bay and Ilha Grande Bay. The sampling procedure involved a Longline farming system, which was used to sample Mussels from a depth of between 1.5 to 2 metres to ensure the results were as accurate as possible (Galvao et al. 3). Over the two seasons the experiment lasted, several samples were gathered on a fixed timetable from all three sites for purposes of comparison. The experiment lasted 8 months, during which it gathered thirty specimens per site for comparison and analysis purposes. Therefore, 6,400 specimens were taken in total, although one must allow for the possibility that this number might not be precise. With such a large number, the scientists were almost guaranteed accuracy since the samples would account for numerous variables.
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