Cholesterol Maintains the Stability and Fluidity of the Membrane
At high temperatures, the membrane starts deforming. But the hydroxyl group of the cholesterol holds the phospholipids tight and prevents the breakdown of the membrane.
Thus, it helps the membrane not to lose its stability. At low temperatures, the phospholipids come closer and start freezing. But cholesterol, with its bulky ring structure, does not allow the phospholipids to crystallize, maintaining its fluidity.
Cholesterol Content Affects the Transport Proteins in the Membrane
Membrane cholesterol affects several membrane proteins, such as ion channels, transporters, and receptors. Cholesterol enrichment inhibits all membrane ATPases. However, increased content of membrane cholesterol stimulates several other membrane transport proteins. An increase in sarcolemma membrane cholesterol inhibits the ATPase action. On the contrary, the removal of cholesterol from the membranes, even in small amounts, causes ATPase stimulatory effect. Increased calcium ion flux through the calcium channel in cell membranes is the result of increased cholesterol content. Within the plasma membrane, cholesterol plays an important role in intracellular transport, cell signaling, and nerve conduction.
Thus, it can be said that an alteration in cholesterol content would influence the function of a cell. A study on the effect of membrane cholesterol could pave the way for studying disorders related to the plasma membrane and finding a way for a cure.
Chemically, cholesterol is a steroid. Many of us believe that cholesterol is bad for our health because of the recent rise in heart-related diseases in human beings due to the deposition of high cholesterol levels in the body. But the interesting fact is that cholesterol in its useful form, position, and required quantity has many important functions. Being a main component in the plasma membrane, it helps maintain the selective permeability and fluidity of the membrane.
A large amount of cholesterol is synthesized in liver cells using Acetyl-CoA, a product of glucose-metabolism. The cytoplasm and endoplasmic reticulum are the sites where a series of reactions occur to make cholesterol, and it is then delivered to the plasma membrane and organelles by the secretory pathway.
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