According to the laboratory tests carried out, individuals with classic MSUD were found to have near-zero leucine oxidation and their loss of branched-chain amino acid through the urine was negligible. It also emerged that individuals with intermediate MSUD had a higher leucine tolerance. They were less prone to significant fluctuations in the branched-chain amino acid than those with classic MSUD. This can be attributed to the existence of BCKAD enzyme activity. They are, therefore, less prone to essential amino acid deficiencies. There is no disparity in the levels of branched-chain amino acids in such individuals, whether they are well or unwell.
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