The results of the experiment demonstrated that the Glycolysis reaction that took place in a warm water bath had the highest volume of carbon dioxide than the test tubes that had been placed in control, cold, and hot water baths as illustrated by figure 4. The results also showed that Glycolysis in warm water took less time to produce the highest volume. In the first ten minutes, the test tube that had been placed in a warm water bath had produced the highest volume of carbon dioxide and the process had stopped after ten minutes which may mean the fermentation process had completed or reached a point where the activity of the enzyme was being affected by the introduction of other limiting factors like the PH. As the rate of fermentation increased, the level of PH continued to decrease meaning the concentration of the acid was increasing until the enzyme maybe was denatured or made inactive through a change of its conformation as illustrated by figure 3 and appendix 1 respectively.
Calculations for the t-test between hot and control water bath was determined to be 0.682, hot and cold-water bath was determined to be 2.453 and hot and warm water was determined to be 2.217. The t-test for the control was calculated to 0.00964, and an average of CO2/minute of 0.1611, the cold water bath t-test was 0.0000 and average CO2/minute of 0.0167, the t-test value of the warm water bath was determined to o.0193 and average of CO2/minute of 0.5556 while the t-test of the hot water bath was calculated to 0.0304 and average volume CO2/minute of 0.2399.
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