Clostridium perfringensÂ gives a negative result on catalase test. Since it grows in absence of oxygen, it obtains energy required for growth by breaking down proteins and from the fermentation of carbohydrates. Aerobic respiration which takes place in bacteria that grow in presence of free air produces a by-product, hydrogen peroxide, which is removed by catalase. The oxygen that is available in organic matter is all consumed by other types of bacteria leaving an anaerobic environment that is conducive for the development of clostridia spores. There is, therefore, no need for removal of hydrogen peroxide by-product by catalase hence this bacterium is catalase-negative. It is also oxidase negative and forms gas and acid from carbohydrates. These bacteria are indole negative as well (Todar, 2009, p. 1).
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