In the introduction, the authors provide an unambiguous background on the use of neural stem cells (NSCs) in treating neural disorders. It is known that astrocytes produce certain substances, which play significant roles in deciding the outcomes of neural stem cells. Neural stem cells can be used in nervous disorders. However, the key challenge lies in maintaining the ability of the propagation of stem cells as well as deciding the type of cells that are formed. The neighbouring microenvironment of the stem cells is thought to play a significant role in influencing the fate of stem cells. Wang et al. (2011) provide numerous examples of previous studies showing the role of astrocytes in the central nervous system. It is shown that astrocytes affect the development of mesenchymal stem cells obtained from bone marrow. Then again, the precise substances and their mode of action in deciding the fate of neural stem cells remain unknown. Therefore, the authors seek to determine whether the effects observed in mesenchymal stem cells are replicated in NSCs. The general experimental procedure is well illustrated. Astrocytes triggered by lipopolysaccharides are used to imitate the effects of activities of astrocytes following inflammatory injury. In addition, the authors provide the implications of their findings on the treatment of neurological disorders. However, the authors do not explicitly state the hypotheses of their investigation. Therefore, it is unclear whether a null or alternative hypothesis is being tested by the outcomes of the investigation.
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