Disc susceptibility test has been one of the effective methods to be used in identification of microorganisms in microbiological laboratories. Antibiotic susceptibility in clinical microbiology is best carried out using the disc diffusion test. The test involves the use of a filter disc which usually contains antibiotic which is applied on an agar plate, Mueller-Hinton agar, which has been already inoculated. If the bacteria are resistant (R) for that particular antibiotic, regions of inhibition will appear on the disc. (FIG.8).
However, if the bacteria is susceptible (S) to the antibiotic in question, there will be no zones of inhibition around the antibiotic disc. AlthoughÂ Staphylococcus aureusÂ is one of the most pathogenic bacteria to man and other animals, it is as well very susceptible to various antibiotics such as Gentamicin, Penicillin and Erythromycin. The aim of this experiment is to determine the susceptibility ofÂ S. aureusÂ to various antibiotics.
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